Firstly, add Culture and UI Culture information to your web.config file.
<system.web> <globalization uiCulture="en" culture="en-GB" /> system.web>
Sett the EnableScriptGlobalization and EnableScriptLocalization to true as properties of the ScriptManager.<cc1:ToolkitScriptManager ID="ToolkitScriptManager1" runat="server" EnablePartialRendering="true" EnableScriptGlobalization="true" EnableScriptLocalization="true"> cc1:ToolkitScriptManager>